By Youjie ZHENG, Shanghai Cheermore Biological Products Co., Ltd, China
Context of the study: The interaction of TAGLN and USP1 can promote ZEB1 ubiquitination degradation in UV-induced skin photoaging, and Zer can be used as an interactive interface inhibitor of TAGLN/USP1 to prevent photoaging. The poor solubility and permeability of Zer can be improved by preparing it as a nanoemulsion, which can effectively prevent skin photoaging caused by UVA in wild-type (WT) mice. Zer cannot effectively resist the photoaging caused by UVA in TAGLN−/− mice because of target loss.
“Characteristics and differentiation of normal human epidermal keratinocytes” by Océane GUYOT , HCS Pharma, France
After one day of culture post-confluence, keratinocytes expressed cellular keratin 14 (green) and actin filaments (red). Nuclei are in blue. The scale bar corresponds to 200 µm.
Context of the study : HCS Pharma studied the differentiation of primary keratinocytes in a monolayer submerged culture. The effect of confluence, culture time and calcium addition were examined. The image was extracted from one of these experiments.
By Caibin ZHU, Shanghai Cheermore Biotech Co., Ltd., China
Context of the study: Phellinus linteus (PL), which is a typical medicinal fungus, has been shown to have antitumor and anti-inflammatory activities. miR-CM1 is a novel miRNA extracted from PL and was found to be resistant to photoaging through cell function experiments. To explore whether miR-CM1 plays a good anti-aging effect at the animal level, the mouse photoaging model was established. The treatment group was divided into the miR-Exo group and FELNVs group. The miR-Exo group was treated with the nanoparticles of HA-PEI loaded with miR-CM1 to simulate the exosomes (“artificial exosomes”). FELNVs were fungi exosome-like nanovesicles extracted from PL. After 28 days, we collected the dorsal skin of the mice to evaluate the effect of miR-CM1 on UV induced collagen content of mouse back skin by Fontana-Masson staining. Result showed that the loss of dense collagen in the dermal layer was significant in the model group, whereas miR-Exo and FELNVs treatment could significantly increase the collagen content.